• Uptake into tissue organs
  (in vivo)

Uptake into tissue organs (in vivo)

We had a strong interest in CTP because of its high absorbability and functionality, and studied the distribution to the tissues after CTP absorbed into the body.

Test for uptake of CTP into the tissues

1) Methods

<Test sample>

As seen in the previous verification of absorption from the digestive tract, Gly-Pro-Hyp, one of the major components of CTP, was prepared as a test sample with tritium labeling(³H)

<Method for ARG>

Male rats at 5 weeks of age after fasting 16 hours were administered 0.35 mmol/kg of the test sample orally, A whole body autoradiogram (ARG) was then created 24 hours after final administration, and the distribution of the test sample to the various tissues was observed.

2) Results

Test results are shown in the figure below. In a whole body autoradiogram 24 hours after a single administration, "radioactivity distribution" - that is, distribution of the test sample was shown especially in skin, bone and cartilage.

Distribution to the various tissues (ARG) *1

Uptake of CTP to joints and peripheral tissues of bone *2

*1 Yamamoto et al. Bioscience, Biotechnology, and Biochemistry 79(12):2026-2033(2015)

*2 Yamamoto et al. Pharmacometrics 89(5/6) (2015) in press

3) Discussion