Promotion of collagen production (in vitro)
Collagen plays a crucial role in the skin, such as in keeping skin turgor. We studied what effect CTP had on collagen production by using a human normal skin-derived fibroblast.
Test for promotion of collagen production
1) Methods
- <Test sample>
- CTP was prepared.
- <Measurement method>
- In cultivated human normal skin-derived fibroblasts, which were cultured in 35 mm dishes in 5% FBS-Eagle MEM for 3 days, it was verified whether the amounts of type I collagen secreted into the culture supernatants were changed or not depending on the addition of ascorbic acid of 1μm (ASA) or CTP of 3 μg/mL. The culture supernatants after cultivation were subjected to a quantitative measurement of type I collagen by the ELISA method using a rabbit anti-human type I collagen antiserum. The quantities of cells in the dishes were also counted.
2) Results
The result of the quantitative measurement of collagen is shown in the figure below. The CTP addition caused an increase in collagen production. Moreover, it significantly enhanced collagen production which was induced by ASA and CTP addition. The numbers of cells were not changed during the test.
The amount of collagen production
3) Discussion
CTP increased the production amount of collagen in human normal skin-derived fibroblasts. Therefore, it is expected that the promotion of collagen production by ingestion of CTP-containing collagen will bring about improvement of bedsores (pressure ulcer) and inhibition/improvement of wrinkles.
